Journal: Pharmaceutical biology
Article Title: Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats.
doi: 10.1080/13880209.2021.1877736
Figure Lengend Snippet: Figure 3. Effects of berberine on key effectors of the TLR4/NF-jB signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.
Article Snippet: The membranes were blocked with 5% skim milk, followed by successive incubations with rabbit anti-mouse TLR4 polyclonal antibody (1:2000, Santa Cruz, USA) at 4 C overnight and mouse anti-rabbit IgG monoclonal secondary antibody (1:10000, Bioss, China) for 2h.
Techniques: Expressing, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Comparison